GrowDex® combo pack

GrowDex® consists of nanofibrillar cellulose (1.5% w/v) and 98.5% water. The nanofibrillar cellulose fibers self-assemble forming a random 3D structure to support cell growth, differentiation, and spheroid formation.

GrowDex hydrogel matrix is supplied ready to use and can be mixed directly with cells and culture media, no additional preparation steps are required. GrowDex is stored and used at room temperature and is compatible with manual and automated liquid handling systems.

To achieve optimal cell growth and differentiation, it is necessary to determine the appropriate concentration of GrowDex and bioactive molecules (e.g., growth factors, extracellular matrix (ECM) proteins, and/or other molecules). Viscosity (i.e. stiffness) of GrowDex can be adjusted by dilution with media or PBS to accommodate culture of a variety of cell types.

Note: GrowDex® is supplied at a working concentration of 1.5 %. It is not a concentrate. GrowDex® can be used in concentrations between 0.2-1.5 %, which enables cell culturing in a microtiter plate.

• The product is supplied sterilized
• 1.5% solution (w/v) of nanofibrillar cellulose
• The pH of a packaged material is neutral
• Opaque, not autofluorescent, suitable for microscopy studies
• Devoid of animal-derived material, endotoxins, and pathogens
• Biocompatible with human cells and tissues
• Supplied in syringe packaging for simple, accurate dispensing
• Storage temperature: 4-22 °C
• Shipping temperature: Room temperature

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• Sterilized, tested and found negative for bacteria, fungi, and mycoplasma
• Cell viability confirmed/tested using HepG2 cells
• Viscosity confirmed by rheology tests

GrowDase™ is a purified mixture of cellulase enzymes that has been specifically developed to breakdown the nanofibrillar cellulose present in UPM’s GrowDex® hydrogel matrix to soluble glucose. The process is simple, enzyme is mixed with the sample and incubated at 37°C until the GrowDex has been reduced to non-toxic sugars. Enzymatic removal allows cells to be recovered from the matrix efficiently, retain their 3D structure, e.g. spheroid or organoid in-tact and does not exhibit any adverse effect on cell viability or functionality.

• Cellulase enzyme mixture in adequeous sodium acetate buffer solution
• Sterile
• 10 mg / ml
• pH 5

Research use only, not to be used for diagnostics or therapeutics.

MSDSs
Material Safety Data Sheet, GrowDex
Material Safety Data Sheet (US), GrowDex
Material Safety Data Sheet, GrowDase

Brochure
GrowDex hydrogel range product brochure

Instructions for use and protocols
Instructions For Use, GrowDexInstructions For Use, GrowDase
Quick Start Guide
Technical Protocols
FAQ
GrowDex calculator